Figure 1. Effect of serum protein concentration on cell attachment

Figure 2. Serum requirement of two ACR and two control cell strains.

Figure 3. Growth rate rank of two ACR (CRL 1533 and CRL 1532) and two control (CRL 1467                and CRL 1471) cell stains at various serum protein concentrations and at their optima as                determined by the Student-Newman-Keuls test.

Figure 4. Serum dose response curves of two normal and two ACR cell strains expressed as                percentages of optimal growth rate.

Figure 5. Effect of various numbers of washes with serum free media on cell attachment.

Figure 6. Effect of two washes with MCDB 104 at various concentrations of serum protein.

Figure 7. Serum requirement of one ACR (CRL 1613) and one control (CRL 1467) cell strain.

Figure 8. Growth rate rank of the ACR and the control strain shown in figure 7.

Figure 9. Serum dose response curves of the ACR strain CRL 1613 and the normal control strain               CRL 1467 expressed as percentages of optimal growth rate response.

Figure 10. Serum dose response curves of 5 ACR and 4 normal control strains.

Figure 11. Column chart showing the growth rates of 4 normal and 5 ACR strains at eight serum                  protein concentrations in MCDB 104.

Figure 12. Growth rate rank of 4 normal and 4 ACR cell strains at 7 different serum concentrations                  and at their optima as determined by the Student-Newman-Keuls test.

Figure 13. Area graphs of the serum dose growth response curves of five ACR and four normal                  control strains.

Figure 14. Average serum dose growth response curves of four normal (pluses) and four ACR cell                 strains (squares) expressed as percentages of their respective average optimal growth                 rate.

Figure 15. Cultural aging of the normal cell strains CRL 1467

Figure 16. Serum dose response curves of the normal cell strain CRL 1467 at two different                  population doubling levels.

Figure 17. Effect of culture age on the shape of the serum dose response curve of the normal cell                  strain CRL 1467. Figure 18. Growth of three normal (right) and four ACR (left) cell                  strains in MCDB 104 containing 2 mg/ml of serum protein.

Figure 18. Growth of three normal and four ACR cell strains in MCDB 104.

Figure 19. Decline in growth rate of 3 ACR and 2 normal cell strains with increasing cell density.

Figure 20. Effect of refeeding cultures with twice the media volume.

Figure 21. Hypothetical growth rate curves fitted to the serum dose response data of CRL 1467                  from figure 2.

Figure 22. Effect of various values of the A, B, and C constants on the shape and height of the serum                 dose response curve generated by the growth rate equation                                            Y = (7.09 X/ (0.80 + X)) -0.72 X.

Figure 23. Hypothetical growth rate curve fitted to the serum dose response data of normal dermal                  fibroblast cell strains.

Figure 24. Hypothetical growth rate curve fitted to the serum dose response data of 4 ACR dermal                  fibroblast cell strains.

Figure 25. Hypothetical growth rate curve fitted to the serum dose response data of the normal cell                  strain CRL 1476 at two different population doubling levels.